|
KMID : 1143420200130231643
|
|
Public Health Weekly Report
2020 Volume.13 No. 23 p.1643 ~ p.1649
|
|
|
Development of a double?antibody sandwich ELISA for sensitive and specific detection of Yersinia pestis
|
|
Choi Sang-Yoon
Jeon Jun-Ho
Kang Byung-Hak
Rhie Gi-Eun
|
|
|
Abstract
|
|
|
Yersinia pestis is a highly pathogenic gram-negative, rod-shaped bacterium that causes plague. Y. pestis is classified as a highrisk pathogen, due to its severe threat to public health and safety. Antibody-based commercial kits for the detection of Y. pestis are available but these kits are not sensitive enough to identify plague patients in the early stage of infection.
Therefore, this study developed a biotin-streptavidin-based sandwich enzyme immunoassay to perform and allow sensitive and specific detection of Y. pestis. In this assay, F1 capsular protein and Y. pestis were captured by anti-F1 mouse monoclonal antibody followed by detection with biotinylated-anti-F1 rabbit polyclonal antibody and horseradish peroxidase- conjugated streptavidin. The F1 sandwich enzyme-linked immunosorbent assay (ELISA) could detect not only the F1 protein up to 17 pg/ mL but also Y. pestis up to 129.2 CFU/mL in human serum. In addition, the F1 ELISA did not show any cross-reactivity with various proteins and bacterial strains. This study concluded that F1 ELISA could be utilized as a tool to help confirm the diagnosis of plague patients.
|
|
|
KEYWORD
|
|
|
Yersinia pestis, diagnostics, F1 capsule, sandwich ELISA
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|